Modeling a Neurexin-3α Human Mutation in Mouse Neurons Identifies a Novel Role in the Regulation of Transsynaptic Signaling and Neurotransmitter Release at Excitatory Synapses The Journal of Neuroscience, 2019 Nov 13
"In vitro molecular replacement with Nrxn3αA687T SS4− but not Nrxn3αA687T SS4+ selectively increases the size of <t>excitatory</t> <t>presynaptic</t> terminals. A, Representative images. B, C, Summary graphs of inhibitory gephyrin (B) and excitatory PSD95 (C) postsynaptic immunoreactive cluster density, intensity and size reveal that excitatory and inhibitory postsynaptic morphology were not altered by either SS4 isoform of Nrxn3αA687T. D–F, Same as A–C except neurons were immunostained with antibodies to detect <t>vGAT</t> (inhibitory) and vGluT1 (excitatory) synaptic markers. Representative images of presynaptic vGAT and vGluT1-immunoreactive clusters (D) and summary graphs of vGAT (E) and vGluT1 (F) cluster density, intensity, and size reveal that Nrxn3αA687T SS4− selectively significantly increases vGluT1 cluster size. Data shown are mean ± SEM; mean values calculated from the average of the number of total experiments. Numbers in bars represent cells/number of experiments. Statistical significance was determined by one-way ANOVA, multiple comparisons. *p = 0.05, #p < 0.05). "